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Introduction: Pathologies of hair growth can be psychologically distressing but they are poorly controlled. Hormones and paracrine factors regulate the hair follicle and its associated glands. However, our understanding of their mechanisms is limited, restricting the development of new treatments for hair disorders. Therefore better treatments for hair loss disorders are required. Some plant extracts are believed to have effect on hair growth. Urtica Cannabina L and other are used traditionally as stimulators of human hair growth, but their effects on hair growth in vivo has not been studied yet. Goal: The aim of this study was to investigate the actual effects of those local plant extracts used as a traditional herbal treatment for hair loss, using in vivo mouse model; and to compare their effectiveness with the best medical treatment available. Material and methods: Effects of extracts from Urtica Cannabina L, and Glycyrrhiza uralensis Fisch both prepared separately and mixed at recommended concentrations. Experimental groups were compared with standard (positive control) and negative control groups. Shaved back of Balb/c mice (4 weeks old) were treated daily for 28 days (four groups, n=6 per group), and degree of their effectiveness was observed and compared with each other and with both of positive and negative control. Results: show that mixture of the two herbal extract have similar significant hair growth promotion effect compared with other groups and negative control. Therefore, extracts stimulates rodent pelage follicles in vivo, thus possible to use as promoter of hair growth. Keywords: Urtica Cannabina L, Glycyrrhiza uralensis Fisch, hair follicle, hair loss, mice.
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Wistar nonlinear rats weighing 170-220g. Rats were divided 5 groups, including control group, group-1, group-2, group -3 and reference group. Dried thistle extract and raw bovine testicle were contained by 1:1, 1:2 and 2:1 ratio. Each 0.1g ratio was dissolved in 20 ml distilled water and administered 2 times per day. Blood sampling was done for each rat after 3, 7, 14, 21, 28 and 35 days. Their testosterone level was measured by ELISA Kit. Results: The results indicated that free serum testosterone level in male rats increase and decrease in 7 days frequency. All tested groups showed gradual increase in the level of free serum testosterone when compared to that of corresponding control (p<0.05). Statistical comparison of all groups revealed that the maximum level was found in group 1. Moreover, group 3 was showed gradually increase in level of free serum testosterone, irrelative with period of decrease testosterone level. Conclusion: According to our results and previous study, it is suggested that preparation with Tribulus terrestris L. extract could be used in the androgen deficiency and erectile dysfunctions. Keywords: Tribulus Terrestris L, Free testosterone, Dihydrotestosterone, Protodioscin
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Introduction: High-performance liquid chromatography is one of the powerful qualitative and quantitative tech-niques, generally used for the analysis of pharmaceutical preparations. HPLC’s virtue lies in its versatility. We can use it to separate compounds of molecular weights from 54-450000Daltons. Amounts of material to be detected can vary from pictograms and nanograms to micrograms and milligrams to grams depends on which kind of detectors used for chromatographically separations. This article was prepared with an aim to review different aspects of HPLC, such as principle, types, and its applications. Keywords: High performance liquid chromatography, applications, mobile phase, stationary phase
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Introduction In recent years we have observed that there are been more and more studies and increased reg- ulatory action regarding animal, plant and mineral-based raw materials, drugs, biological prod- ucts, groceries and food products.Therefore, dehydrated cow bile liver hydrolisate appears less harm- ful for the human body and may minimize damage to liver cells, have regenerative and healing properties, and may support the healing / recovery process process. It is important to find and apply preparations that work against acute inflammation of the liver protein, fat and carbohydrate me- tabolism. Pharmacological research was undertaken with the performance of a histomorphological assessment with reference to the hydrolisate liver, dehydrated cow bile, silymarin 3 with a composi- tion containing “Sillichol”, determining how it seriously affects the inflammation of the liver operation. Goal: To determine the presence of the liver tissue morphology with reference tothe investigational / experimental new drug “Sillichol”. Material and Methods: Male Wistar rats, specified as pathogen-free, weighing 200-250 g, were obtained from the Vivarium of the Department of Pharmacology, Drug Research Institute, and were used for the chronic CCL -induced liver injury model in all experiments. Eighteen rats were di-vided into three groups (with each group consisting of 6 rats).The rats were sacrificed at the end of the 14 days of the investigation, and the livers were im- mediately removed (Methods R.Virchow). Liver slices were made from a part of the left and cen- tral lobes, and immediately fixed in 10% buffered formalin phosphate solution, embedded in par- affin, and cut into 5μm sections. Subsequently, the sections were stained with hematoxylin and eosin (H&E) and observed under a microscope to evaluate histopathological changes (20x20). Result: Liver tissue sections of the rats were stained with H&E. The histopathological assessment in the livers was performed for all groups. Rats in the negative healthy group exhibited normal, well- defined histological structures, without any signs of vascular or inflammatory changes: no cavita- tions, necrosis or fibrosis were found in normal control sections. The histopathological analysis of the livers revealed signs of toxicity after administration of CCl .This toxicity was significant in comparison with the control group and cavitations, fibrosis in board ar- eas, mild vascular congestion and moderate inflammatory changes with congested sinusoids, nu- clear changes, and centrilobular necrosis. Sinusoids spaces were flooded with inflammatory cells. The Sillichol-treated animals of the experimental group showed a complete reversal of toxic ef- fects in the liver cells; no necrosis was seen. The central vein and portal triads appear normal and show an increase of Kupffer cells. Some of the hepatocytes indicated binucleation, suggesting re- generative activity with feathery degeneration of hepatocytes.The Carsil-treated animals of the standard group: The histology of the liver sections in rats adminis- tered with Carsil indicated significant improvement with less damage of liver tissue, as indicated by a reduced level of necrosis, narrow fibrotic septae, fat storing cells, Kupffer cells, and narrow cavita- tion. Conclusions We found that the “Sillichol” biological active product treatment reduced hepatic necrosis and fibro-cal active product improved the regeneration process of liver cells, helped to normalize cell struc- ture, and had an effect on the anti-inflammatory action in damaged liver cells. Keywords: Histology, Carbon tetrachloride, Liver damage, Silichol, Liver cell
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Introduction: The present study shows the processing of the pharmacopoeia article for the preparation of Di- clofenac sodium extended release matrix tablet formulated from alternative characteristic poly- mer. The matrix tablets were prepared according to traditional method of the wet granula- tion for tablet form medicine. Method: A standardization study made use of several international pharmacopoeias, such as the Mongolian National Pharmacopoeia (MNP 2011), United States Pharmacopoe- ia (USP 32), Chinese Pharmacopoeia (CP 2010) and U.S Department of F.D.A for the sub- stance’s requirements. Result: The results from this study showed a completely processed manufacturing pharma- copoeia issue of the Diclomon 100 mg retard tablet and its technological and standardized re- quirements.
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"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."
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Introduction: Factors are increasing in Mongolia, which is producing food product using sea buckthorn berry. However, those factors use pulp oil, juice and throw skins, pulp and seeds. Those solid remains named pomace. The pomace contains many kinds of vitamins, trace elements and other biologically active substances including fat soluble vitamins /А, Е, К/, fatty acids / linoleic, linolienic, oleic/, carbohydrates, water soluble vitamins /С, В1, В2, folic acid/, flavonoids, carotenoids. Therefore, pomace is usable as raw material for biological active product. Goal: The aim of study is to invent sample of biological active product including seabackthorn pomace Materials and methods: The seabuckthorn pomace was provided by Monos Foods Co Ltd. Ascorbic acid and other expient were provided by Drug Research Institute. Sea buckthorn (H. rhamnoides) pomace was dried by Labconco freezone L12 freeze dryer in Drug Research Institute. Moisture and total ash were determined by method according to MNS 5225:2002, contents of total fat was determined by Soxhlet method, vitamin C and total acidity were analyzed by titremitric method, total flavonoid was analyzed spectrophotometric method and impurity of dry pomace was measured by The State Pharmacopoeia of the Russian Federation XI. Quality requirements of tablet, capsule and granule were determined by Mongolian National First Pharmacopeia. Total bacteria and fungal were determined by method in MNS 5194:2002 and MNS 5193:2002. Results: Dried seabuckthorn pomace was standardized according to MNS 5225:2002. The results indicated that dried seabuckthorn contains humidity 4.3%, total ash 2.16%, β-carotin 110.3%, fiber 31.78%, total flavonoid 0.25%, impurity 0.13%, acidity 2.06%, total fat 33.3%, total fungi count 3*102 and bacterial count 2*102, respectively. We created tablets 0.3 g contain dry pomace and determined quality and quantity parameters. Quality requirements of tablets were standardized and following results were obtained: the 0.3g tablet had an average weight 0.301g, the dissolution of the 0.3g tablet was 97.6%, disintegration 2.93 minute and ascorbic acid 30.66 mg. Furthermore, capsules contain dry pomace were created. Its average weight was 0.45g ±7.5%, disintegration 24 minute and β-carotine 0.12 mg, respectively. Finally, granule product contains vitamin C and dry pomace was created. In this granules content β-carotene 1.32 mg and vitamin C 145 mg, respectively. Conclusion: Quality requirements and microbial contaminants of dry seabuckthorn pomace were agreed with MNS 5225:2002. 0.45 g capsule, 0.3 g tablets, 5g granule were created. Those new products meet the general requirements for tablet; capsule and granule by Mongolian National First Pharmacopeia, and 0.45g capsule, 5g granule products were more suitable for production of biological active products. Key words: sea buckthorn pomace, (H. rhamnoides), biological active product
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Introduction: Constipation is not often regarded as a major therapeutic issue, but the use and abuse of laxatives by older people is highly prevalent. It is important for clinical to have an understanding of constipation to people, and of ways to improve laxative use. The laxatives most appropriate for older people include stimulants such as senna, bulking agents. Cassia acutifolia L and Rheum undalatum L have been used as a laxative drug in Asian traditional medicine for a long time. The pods and leaves contain antraquinone aglycons and antraquinone glycosides which are laxative form while rhein is a major component. The anti constipation ability of combinations of plant preparations consisting of Cassia acutifolia, Rheum Undalatum, Terminilia Chebula Retz, and soda were studied. Anti constipation drug set for LD50 samples of. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of Shinshilla species weighing 2000-2200 grams, 60 mouse of Balb/c species weighing 20-32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Goal: The aim of study is determination anti constipation activity in plant preparations. Material and methods: Rheum undalatum L was collected from Medicinal botanical garden of Drug Research Institute in September of 2011. Cassia acutifolia, Terminilia Chebula and Soda were to be out from Monos food LLC in August of 2013. Maceration was chosen by suitable extraction method and optimal extragent was ethanol. Antraquinone was determined in evaporated extract of Cassia acutifolia, Rheum undalatum using the method by Russian Pharmacopeia. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of shinshilla species weighing 2000 -2200 grams, 60 mouse of Balb/c specie weighing 20 -32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Result: Liquid extracts were obtained from Cassia acutifolia L, Rheum undalatum L with maceration method. Liquid extracts were evaporated on Vacuum rotor. Sample 1 was have anti constipation activity Keywords: Cassia acutifolia L, Rheum undalatum L, Terminilia Chebula Retz, salt, rabbits, rats, mice, and Constipation
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The world pharmaceutical market will reach nearly USD 1,200 billion by 2016, an increase from the USD 956 billion recorded in 2011. The 10 largest drug companies control over one-second of this market. The drug companiesinvestinhigh cost of promotion, and it uses a variety of promotional strategies to influence physicians’ prescribing decisions. Within this context, medical representatives are the key personnel employed in promoting their products. For example in the USA, drug companies spend $54.5 billion on marketing promotions and they spent more than US$10 billion on representative cost. In Mongolia, medical representatives of foreign countries are increasing over the years. So doctors and pharmacists often receive medicine information from medical representative. But the research about medical representative influence on physicians is not observed. The purpose of the study is evaluating pharmaceutical marketing promotions tended to family health centers/clinics physicians. Random sectional, organized based survey with questions was carried out of physicians of the family health centers/clinics. SPSS-20 and Microsoft office excel programs were used to colligate and processing information. Result, discussion: In 2012 the Libya, an anonymous survey questionnaire was circulated to 1,000 Libyan doctors in selected public and private practice settings in Tripoli, Benghazi and Sebha. A questionnaire return rate of 61% (608 returned questionnaires) was achieved. Most respondents (94%) reported that they had been visited by medical representatives at least ‹once› in the last year. Fifty per cent of respondents met with medical representatives at least once a month, and 20% at least once a week. In 2010 the Germany, 100 physicians in each of three specialties (neurology/psychiatry, general medicine, and cardiology) were surveyed with a questionnaire containing 37 questions. 208 (69.3%) questionnaires were anonymously filled out and returned. The result was 77% (n = 160) of all physicians were visited by PSR at least once a week, and 19% (n = 39) every day. In this study, an anonymous survey questionnaire was circulated to 100 physicians of the family centers/ clinics. A questionnaire return rate of 99% (99 returned questionnaires) was achieved. Most respondents 100% reported that they had been visited by medical representatives at least once in the last years. 55.5% of respondents met with medical representatives at least once a month, so 24.2% at least once a week. So 76.8% questionnaireswere satisfied after having representative meetings are informative, advertising and information include equally among 57.2 questionnaires had complete answers. Conclusions • All respondents met with medical representatives at least once a month. • Content of representatives meetings are equally adverting and informative so it influences positively to them. • 61.2% questionnaireswere satisfied after having representative meetings.
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Introduction: Achillea asiatiсa has been used in traditional medicine for one hundreds of years. Many compounds have been isolated from /Achillea asiatica/, including flavonoids, sesquiterpenes and essential oils. Salsola collina herb grows in Siberia, Baikal and Altai. It contains a variety of amino acids, flavonoids, glycosides, and vitamins. It has a positive effect on metabolism of fats in liver, regulates cholesterol and sugar in the blood and is recommended for weight reduction. Salsola collina is widely distributed in droughty and semi-droughty area, which is used as a kind of folk remedy in traditional Chinese and Mpngolian traditional medicine for treatment of hypertension and liver protectevity . Goal: The aim of study is to choose optimal extragent and determination liver protecting activity in Achillea asiatiсa, Salsola colina and to determine main substances in these herbals. Material and Methods: Achillea asiatiсa was collected from Dorgonot mountain Tuv province in June of 2012, Salsola colina were collected from Medicinal botanical garden of Drug Research Institute in August of 2012. Maceration was chosen by suitable extraction method and optimal extragent was ethanol. Flavonoids were determined in evaporated extract of Achillea asiatiсa, Salsola colina using the method by Mongolian National first Pharmacopeia. We use non line wistar rats, determination liver protecting activity biochemical analysis by Hospitex analyzer. Result: Liquid extracts were obtained from Achillea asiatiсa, Salsola colina with maceration method. Liquid extracts were evaporated on Vacuum rotor. The tehnological parameters of liquid extracts are the main important index to calculate the extragent correctly, to establish the material balance exactly, and to extract the process efficiently. 70% ethanol (1:10 sample/extragent) was determined by optimal extragent for Achillea asiatiсa, 80% ethanol (1:10) for Salsola colina. Achillea asiatiсa, Salsola colina were have liver protecting activity. Conclusion: Some technological parameters of liquid extract were determined such as optimal extragent and the effective extracting method of Achillea asiatiсa, Salsola colina. As the result of determining these parameters, it has much practical significant for establishing the technological condition to extract the biological activity compounds completely from their plants. Key words: Achillea asiatiсa, Salsola collina, technological pharameters, flavonoid, anthraquinons, liver protecting activity
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Introduction: Normal human body temperature generation increase by called fever. Various disease syndrome symptoms of fever and high temperature ( 38.50C or more ) reduce the short term, especially when young children cerebral glucose deficiency epileptic disease is beginning fall, and brain tissues and cell malnutrition become parallel, paralysis, as a result of Poor memory and create the risk. Therefore, The World Health Organization (WHO) according 0-3 month infants 380C more than, 3-6 months 38.50C, and 6 months - 1 year old children body temperature 390C more than they must to use medicine or called to doctor. Infants and young children’s drug therapy is recommended for use in practice “ParaDenk 125mg, ParaDenk 250mg”, Panadol suspension (contain 120 mg paracetamol), Nurofen (contain Ibuprofen100mg) syrup. Irritation and fever, all the scenes prostoglandin much as synthesized. The main of this ibuprofen’s mechanism is inhibit prostoglandin synthesized. Domestic pharmaceutical market the ibuprofen containing Nurofen 100 ml suspension drugs are widely used in the treatment of young children, but the cost is high. The scope of research and ibuprofen suspension technologies pharmaceuticals manufacturer has been developed pharmacopeias article. The main aim of this study was to perform quality determination of “Ibumon 100mg /5 ml” 100 ml suspension. Material and methods: We studied “Ibumon” suspension technological and tandardization research in Drug experiment and manufacturer sector in Drug Research Institute. We prepared “Ibumon” suspention by Nurofen suspension’s drug registration according to the physical characteristics of materials. The appearance and suryp’s physical and chemical method determined by in the Mongolian national pharmacopeia. The microbial limit test in the Mongolian National Pharmacopeia was performed to determine microbial purity. Suspension’s Ibufrofen content determined by HPLC equipment . Result: “Ibuprofen” suspension pharmaceutical technology research as effective ingredients have been determined. And the suspension active ingredient quantitative analysis determined by HPLC.
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Introduction: Number of kidney acute and chronic disease is increasing rapidly in the world and becoming the major cause of death even population employment capacity is invalid. Statistical report of Mongolian Ministry of Health last 5 years statistic kidney disease is in the 3rd of non contagious disease.Synthetic and chemical medicines used for this sort of disease would have side effects in some cases. Plants, animals and minerals biologically active substances, side effects need to produce new drugs, has attracted the attention of researchers. Goal: identifying pharmacology act of new granule medicine preparation. Materials and Ìethods: Experiment is on 4 kinds of 35 white rat, 150-280 gram WISTAR RAT. 5 rats from each kind. 1. Healthy group 2. Disease model group /Kanamycin+distilled water/ 3. Standard group / Kanamycin+nefromon/ 4. Experiment group / Kanamycin+ +new form of granule medicine/ Kidney disease model was created artificially kanamycin sulfate (Monodoev. A.J, Lameza.S.B, Bartonov. EA 1988) MDA is identified by an amount of concentration and method. (Stalinaya.I.D 1977) Result: Creatinine amount of disease model group of kidney illness created by kanamycin sulfate is compared with healthy group animals and 1.64 times, carbamide amount is 4.25 times, rest of the azotes 2.73 are increased and comparing the experiment group creatinine amount is 1.65 creatinine amount is 1.65 decreased comparing with disease model group. Conclution: When compound ingredients preparation creates experiment animal kanamycin sulfate oxidant dominates, intensify the kidney cell active, decrease the carbamide and creatinine and decrease the kidney cell necrosis. Key words: Kanamycin, Wistar rate, Iris Tenuifolia, Oxytropsis pseudoglandulosa, Ribes Diacanthum and Granule.
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Abstract: Quality study of Inusal preparation herbal combined ingredients with (Salsola laricifolia Turcz.ex litv and Inula helenium L. (1:1)). The quality study of biological active substances of the preparation was identified using standard substances by thin layer chromatography (TLC). As a result of Inusal study by chromatogram appeared the same as standard substances rutin, quercitin brown yellow, inulin dark brown, alantolactone and isoalantolactone blue and pink spots. Therefore, Unusal preparation contains flavonoid, coumarin, sesquiterpene lactones. Keywords: Inusal, Salsola laricifolia Turcz.ex litv, Inula helenium L.
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Introduction: This plants distributed about 2500 species in worldwide and 69 species of those grow in Mongolia. In case of medicinal application of this plant is commonly used traditional medicine in European and Asian. For example, the different preparations of Astragalus mongholicus root, the most commonly used Astragalus species in Chinese medicine. Other Astragalus species which are being investigated for their anti-cancer properties are the Turkish species, and include Astragalus brachypterus, Astragalus cephalotes, Astragalus microcephalus and Astragalus trojanus. Nowdays, this plant source from nature is decreased and areal square de-escalated in Mongolia. Goals: Our main goal was conserves domesticates and improves plant genotypes of local wild medicinal specie and provides raw plant material for scientific testing from the cultivation site without depleting wild sources. Including that main goal we put a purpose of the make comparative study of vegetative organ’s anatomy morphological study on wild and planted type. Materials and Methods: The wild Astragalus mongolicus bunge was collected from Bayanchandmani sum, for Tuv province. Cropped plant was grown medicinal botanical garden of Drug research institute, Monos. Anatomy structure of vegetative organs was identified by plant anatomic method. Result: The article presents the data on structure of vegetative organs of cultivated Astragalus mongolicus Bunge and compared with wild type. Comparative studies were made on the leaf, root, stem and flower and the anatomical structure of vegetative organs of Astragalus mongolicus Bunge and wild type, which were the similar plants on botany and had the great value of medicine. The results indicated that the anatomical structures of their vegetative organs were basically the same, but there are some obvious differences on leaf and stem it depended from geographical influences. Planted plant in particular could alter their environment by affecting light and water avialablity, soil nutrient availability due to uptake. Discussion: Tserenkhand.G was studied Astragalus mongolicus root anatomy in 2003. Our root anatomy study was same to Tserenkhand.G. Conclusion: The results from this study, we are concluded that, the cultivated Astragalus mongolicus Bunge is greatly cultivated in our Botanical garden. Key words: cultivated, anatomy, epidermis
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BACKGROUND: Fourteen species of the genus Iris L is grown in Mongolia. Iris L has been in traditional Mongolia medicine for treatment of urinary and kidney disease. We investigated three species of Iris L such as Iris Bunge /Iris Bungei Maxim/, Iris Tigrida /Iris Tigrida Bge / and Iris lactea /Iris Lactea Pall/ which contains rich amount of biological active substances[1,2]. Urine was collected at tubes during 4 hours, since given experimental and standard preparations orally and compared with control group. As experimental results, the urine levels of treatments groups increased 1.13 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.04 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L.\Z TOOLS AND METHODS: Adult Balb/c mice, each in the weight range of 20-28g, were obtained from the Vivarium, Drug research institute. The animals divided into five groups of four animals each and kept in cages and housed under standard conditions of temperature, humidity and dark light cycle (12h-12h). Diuretic activity was determined by the following methods of Trendelenburg et al (1980). Before experiment, all the animals received priming dose of 0.9% sodium chloride solution (0.3 ml/20g) and the treated groups were given 0.1ml/20g extracts of Iris L. The standard groups were given 0.1 ml/20g of “Nefromon. Immediately after respective treatments the animals were placed in metabolic cages and urine was collected in a measuring cylinder till 4 hours. RESULTS AND CONCLUSION: Results are shown in the ethanol extract of the whole plant of Iris Tigrida Bge at a dose of 0.1ml/20g observed diuresis during the 4 hours of the test (Tigrida Bge 1.0±0.37 ml versus control 0.55±009 ml, whereas in case of standard Nefromon the volume was found to be 0.63±0.08 ml, p<0.05). As experimental Iris L, results, urine levels of treatments groups increased 1.04 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.05 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These result, indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. KEY WORDS: Diuretic, medicinal plants, Trendelenburg methods, flavonoids
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Introduction: Chamaenerion angustifolium (L.) Scop. has a cup-shaped nectary which locates in hollow receptacle, belonging to receptacle nectary. One layer of eqidermis on which modified stoma lie is covered by a thin cuticular layer. The nectary is differentiated from the srperfical layer cells of receptacle and that no special initial has been found. Goal: The aim of this study was to develop a natural Chamaenerion angustifolium (L.) Scop plant’s standardization requirements. Material and Methods: The root samples of natural Chamaenerion angustifolium (L.) Scop was collected from Umnudelger sum, Khentii aimag in August, 2013. The plant material dried under shade at room temperature. Then passed through 120 mesh size to remove coarse powder and fine powder was used for estimation of biologically active compound content in plant material [1, 3, 7]. Shimazdu UV –VIS Spectrophotometer was employed for all spectroscopic measurements using a pair of matched quartz cells and Shimadzu High Perpormance Liquid Chromatography equipment equipped with SPD - 20 A UV detector, CMB - 20 A system controller, CTO-10 AS vp column oven with injector and LC-6AD pumps. Gallic acid, Folin-Ciocalteau, sodium carbonate, methanol, sulfuric acid, acetic acid and glucose used were of the highest commercially available purity [8. 9, 10]. Results: The present study was carried out to develop a simple, rapid, sensitive, accurate, precise spectrophotometric and HPLC method to determine polysaccharide, polyphenolic and gallic acid by simultaneous estimation of in standardization formulation. Chromatographic analysis was carried out by Luna C18 (2) 100A reversed phase column (150 x 4.6 mm) packed with 5μm diameter particles. The mobile phase was 0.1% acedic acid:Methaol (95:5 v/v). The mobile phase was filtered through a 0.45 μm membrane filter. Then it was degassed ultrasonically prior to use. HPLC identification of standard gallic acid was at 278 nm. Flow rate and injection volume were 0.8 ml /min and 20 µl, respectively. Gallic acid was eluted with retention times of 8.2 min respectively. Amounts of gallic acid were 0.3% in plant. The standard deviation values were satisfactorily low and recovery was closed to 100% indicating the reproducibility, accuracy and precision of proposed method. The natural plant contents of the polysaccharid and polyphenolic was found 2.65% and 4.59%, respectively. Conclusion: The results from this study, we developed natural Chamaenerion angustifolium (L.) Scop. plant’s chemical ingredients for the its standardization. Keywords: Chamaenerion angustifolium (L.) Scop., polysaccharide, polyphenolic and gallic acid.
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Introduction: The development of a plant derived supplement with mild effects and reduced toxic and side effects for the treatment of respiratory disease is very crucial. Therefore we tested the cough suppressant effects of three plant preparations that are made from Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisch and Vaccinium Vitis idea/, plants that are common in our country. Materials and Methods:The research was conducted in 2013-2014 at the Pharmacological Laboratory of the Drug Research Institute. The experiment was carried out on four groups of 20 guinea weighing 300-400 grams and the experimental condition of coughing was generated using the method of Ucelay et al. Result:The results of the study show that the combination 3 (Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisc, Vaccinium Vitis idea) had suppressed the most when compared to the other combinations.
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Introduction: The World Health Organization (WHO) according respiratory allergic diseases, nasal mucosal disease , which is one factor to create asthma and more than 300 million people become infected is accured in 2012[1]. In 2012, acconding to statistics of the Ministry of Health for our country was noted of 5% of the total population of the allergic disease and astma.Also In 2000, the prevalence of astma among the population was 2.4%, but in 2010 increased by 4.8%. Accordingly , the prevalence of the disease is increasing year by year, that’s why the imported new drug’s of diseases in the market and the need to produce domestically. Domestic market, we used allergic rhinitis, mucosal inflammatory treatment cholorphenamine 4 mg (Monos Industry LLC), Tavegil (Novartis Russia ), ketotifen (So pharma, Bulgaria), letizine 10 mg (KRKa, Slovani), Cetirizine 10 mg (Bertecs pharma, Russian). Letizine 10 mg, Cetirizine 10 mg drugs are contain of Ceterizine dicholoride, widely used in the treatment of foreign drug. Divided in the medical treatment of children aged 2-6 years , these drugs are expected to give a child , so we import the drug entering the appropriate doses of the company with the goal to obtain. For that purpose we determination allergon tablet’s technological process. Conclusion: Technological experiment “Alergon 5 mg ” tablets were selected ingredients of ceterizine dihydrocholoride 0.005 g, glucose monohydrate 0.09444 g, K-30 0.00006 g, magnesium stearat 0.0005 g. According to our study, ”Allergon 5 mg” tablets average weight, weigth range, disintegration, hardness, assay meets the standards set in the Mongolian National Pharmacopeia requirement of “Tablet drug ”.
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Introduction׃The Ellipin, prepared from bovine liver is a newly developed anticancer agent containing several important fatty acids. Goal: To investigate effects of Ellipin to proliferation assay of hepatic cancer cell function. Materials and Methods: This study was conducted in the Laboratory of Molecular Biology, Institute of Biology, MAS, The Ellipin was developed in the Drug Research Institute of Monos group. HepG2,HCC,23132/87,MDCK,cells lines and the primary liver cancer cells(PCC)we re used for proliferation assay. Results: The results of proliferation assay showed that the ellipin decreased the proliferation activity of HepG2 and PCC cells depending on concentrations: in 50µg/ml 2-3 times, 250 µg/ml fully stopped cells divisions. Conclusions: Our results showed that the Ellipin suppresses HepG2 cancer cell proliferation Key words: Ellipin, HepG2, cell lines, cell proliferation. Figures 4, References 4
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Introduction:The main purpose of health organizations, hospitals and pharmaceutical assistant service organizations are supplying active, efficient and high quality estimation for a treatment, reasonable priced, safe and guaranteed drugs to the people and the health organizations constantly. Digestive system especially peptic ulcer which is one of the disease and its occurrence is high in the population and might be increased further . The reason of death caused by peptic ulcer which takes fourth place in the five prior reason of death . Statistics of digestive organ system’s hospitalized disease is 92363 including 3218 peptic ulcer disease in Mongolia and its 1533 incidence was registered in Ulaanbaatar by 2010. Goal of study: The aim of the study was to determine items and real usage of drugs which are used in the stationary treatment of patients who are suffering from peptic ulcerin the II,III hierarchy hospitals of Ulaanbaatar and within the propose of determining demands and real usage of main drugs for treatment of peptic ulcer especially and sickness rate of digestive system and its occurrence is high in the population of Mongolia. Objectives of the study:Identify disease prognosis and disease genetic from the health statistic report of digestive organ system disease which is most common in population particularly peptic ulcer. Identify the concrete list of drugs which is used for peptic ulcer patients who is having stationary hospital treatment in the II,III hierarchy hospitals in Ulaanbaatar, Mongolia. Conclusion: The major causes of peptic ulcer are stomach acid increasing and contagion. Infection with a type of bacteria called Helicobacter pylori. Purpose of the research work is reveal the drug consuming of treatment eradication Helicobacter pylori, determine up and down trend of the peptic ulcer and suggest the proper usage of drug. Additive or synergistic effects in eradication of H.pylori have been produced by combining two antibiotics with either bismuth ranitidine citrate or proton pump inhibitors.European consensus is that although many options are available, the emerging treatment of choice is seven-day proton-pump-based triple therapy with either omeprazole or Lansoprazole plus two antibiotics (any two of clarithromycin, metronidazole and or amoxicillin). From the survey III hierarchy hospitals didn’t use latest antibiotic clarithromycin and proton pump inhibitor , bismuth, gastroprotecting and treatments pantoprazole, famotidine, quamatel, venter, denol, sucralfate, zantac and zapazid. The II,III hierarchy hospitals used the above mentioned drugs in short terms by few amounts. The cause of not using expensive potent pharmakinetic drugs not often is hospital budget limit and not registered Mongolian drug registrations and not penetrated market. 28 types of main drug were used in the treatment of patients who were suffered from peptic ulcer and treated in the hospital. So 78448.00 thousand tugrik will be spent in the treatment of peptic ulcer. Key word: peptic ulcer,disease, helicobacter pylori, main drug, drug use